Table 3.
Effects of in ovo feeding of γ-aminobutyric acid and embryonic thermal manipulation on hatchability parameters
| Parameters | Treatments1) | SEM | p-value | |||
|---|---|---|---|---|---|---|
|
| ||||||
| CON | G10 | TM | G10+TM | |||
| Number of fertile eggs | 48 | 48 | 48 | 48 | NA | NA |
| Number of hatchlings | 46 | 43 | 45 | 41 | NA | NA |
| Hatchability (%) | 95.8 | 89.6 | 93.7 | 85.4 | 1.9 | 0.255 |
| Hatchling weight (g) | 40.9 | 40.4 | 39.7 | 40.0 | 0.23 | 0.296 |
Hatchability was calculated based on n = 8 repetitions per treatment.
SEM, standard error of the mean; NA, not applicable.
1)
The treatments are described as follows: CON, control eggs without in ovo injection and incubated at standard temperature; G10, eggs injected at 17.5 days of incubation with 0.6 mL of 10% γ-aminobutyric acid dissolved in distilled water; TM, thermally manipulated eggs exposed to 39.6°C for 6 h daily from embryonic day 10 to 18; G10+TM, eggs that received both previous treatments during incubation.