Table 8.

Results of planned contrasts of hepatic gene expression analysis (p-value)

Genes	Planned contrast1)
	CON vs G10	CON vs TM	CON vs G10+TM
SOD	NS	NS	NS
CAT	NS	NS	0.046
GPx1	NS	0.092	0.006
NOX1	0.001	0.019	0.007
NOX4	0.077	NS	NS
ACC	NS	NS	NS
FAS	0.065	NS	NS
NRF2	0.039	NS	NS
EXFABP	NS	NS	0.087
PPAR-γ	NS	NS	NS

¹⁾The treatments are described as follows: CON, control eggs without in ovo injection and incubated at standard temperature; G10, eggs injected at 17.5 days of incubation with 0.6 mL of 10% γ-aminobutyric acid dissolved in distilled water; TM, thermally manipulated eggs exposed to 39.6°C for 6 h daily from embryonic day 10 to 18; G10+TM, eggs that received both previous treatments during incubation. Chicks obtained were grown for 28 days.

SOD, superoxide dismutase; NS, not significant; CAT, catalase; GPx1, glutathione peroxidase 1; NOX1, nicotinamide adenine dinucleotide phosphate oxidase 1; NOX4, nicotinamide adenine dinucleotide phosphate oxidase 4; ACC, acetyl-CoA carboxylase; FAS, fatty acid synthase; NRF2, nuclear factor erythroid 2-related factor 2; EXFABP, extracellular fatty acid-binding protein; PPAR-γ, peroxisome proliferator-activated receptor-gamma.