TABLE 5.
The 46 flavonoids of Muying‐1 chickpea using the internal standard method (IS method) by UPLC‐QqQ‐MS (ultra high‐performance liquid chromatography coupled with triple quadrupole mass spectrometry)
| Number | Compound | CAS | Molecular formula | Retention time (min) | Flavonoid content (ng/g) |
|---|---|---|---|---|---|
| 1 | Silibinin | 22888‐70‐6 | C25H22O10 | 6.85 | 1.384 |
| 2 | Puerarin | 3681‐99‐0 | C21H20O10 | 3.43 | 0.563 |
| 3 | Quercetin | 117‐39‐5 | C15H10O7 | 6.99 | 14.627 |
| 4 | Genistein | 446‐72‐0 | C15H10O5 | 7.13 | 12.056 |
| 5 | Apigenin | 520‐36‐5 | C15H10O5 | 7.08 | 8.369 |
| 6 | Baicalein | 491‐67‐8 | C15H10O5 | 7.54 | 8.544 |
| 7 | Icariin | 489‐32‐7 | C33H40O15 | 6.24 | 0.254 |
| 8 | Baicalin | 21967‐41‐9 | C21H18O11 | 7.13 | 6.779 |
| 9 | Rutin | 153‐18‐4 | C27H30O16 | 4.31 | 74.046 |
| 10 | Hesperetin | 520‐33‐2 | C16H14O6 | 7.37 | 1.434 |
| 11 | Troxerutin | 7085‐55‐4 | C22H34O3 | 9.92 | 705.812 |
| 12 | Hesperidin | 520‐26‐3 | C28H34O15 | 5.04 | 53.149 |
| 13 | Cianidanol | 154‐23‐4 | C15H14O6 | 16.2 | 8.122 |
| 14 | Protocatechualdehyde | 139‐85‐5 | C7H6O3 | 3.11 | 2186.378 |
| 15 | 3,4‐Dihydroxybenzoic acid | 99‐50‐3 | C7H6O4 | 2.26 | 121.811 |
| 16 | Kaempferol | 520‐18‐3 | C15H10O6 | 9.3 | 17.026 |
| 17 | Naringenin | 480‐41‐1 | C15H12O5 | 7.09 | 15.151 |
| 18 | (‐)‐Epigallocatechin gallate | 989‐51‐5 | C22H18O11 | 3.71 | 2.167 |
| 19 | Luteolin | 491‐70‐3 | C15H10O6 | 6.31 | 48.126 |
| 20 | Myricetin | 529‐44‐2 | C15H10O8 | 7.09 | 6.218 |
| 21 | Diosmin | 520‐27‐4 | C28H32O15 | 4.94 | 0.656 |
| 22 | Dihydromyricetin | 27200‐12‐0 | C15H12O8 | 3.45 | 41.169 |
| 23 | Taxifolin | 480‐18‐2 | C15H12O7 | 4.23 | 64.036 |
| 24 | Morin | 480‐16‐0 | C15H10O7 | 5.91 | 3.278 |
| 25 | Fisetin | 528‐48‐3 | C15H10O6 | 6.31 | 47.219 |
| 26 | Chrysin | 480‐40‐0 | C15H10O4 | 8.91 | 0.438 |
| 27 | Neohesperidin | 13241‐33‐3 | C28H34O15 | 5.03 | 20.252 |
| 28 | Myricitrin | 17912‐87‐7 | C21H20O12 | 4.47 | 454.089 |
| 29 | Isoliquiritigenin | 961‐29‐5 | C15H12O4 | 7.78 | 1.950 |
| 30 | Hyperoside | 482‐36‐0 | C21H20O12 | 4.46 | 457.233 |
| 31 | Tangeretin | 481‐53‐8 | C20H20O7 | 9.67 | 5.744 |
| 32 | L‐Epicatechin | 490‐46‐0 | C15H14O6 | 7.23 | 636.388 |
| 33 | Taxifolin 3‐o‐rhamnoside | 29838‐67‐3 | C21H22O11 | 0.35 | 653.933 |
| 34 | Phloretin | 60‐82‐2 | C15H14O5 | 7.02 | 1.326 |
| 35 | Daidzin | 552‐66‐9 | C21H20O9 | 3.95 | 3193.189 |
| 36 | Isorhamnetin | 480‐19‐3 | C16H12O7 | 8.59 | 673.082 |
| 37 | Genistin | 529‐59‐9 | C21H20O10 | 10.1 | 1000.194 |
| 38 | Biochanin A | 491‐80‐5 | C16H12O5 | 9.27 | 1157.400 |
| 39 | Glycitin | 40246‐10‐4 | C22H22O10 | 4.07 | 15.759 |
| 40 | Quercitrin | 522‐12‐3 | C21H20O11 | 4.97 | 2.833 |
| 41 | Vitexin | 3681‐93‐4 | C21H20O10 | 4.38 | 1.751 |
| 42 | Artemisinin | 491‐54‐3 | C16H12O6 | 9.18 | 1.778 |
| 43 | Glycitein | 40957‐83‐3 | C16H12O5 | 6.22 | 12.174 |
| 44 | Astragalin | 480‐10‐4 | C21H20O11 | 4.91 | 549.015 |
| 45 | Acacetin | 480‐44‐4 | C16H12O5 | 6.24 | 512.189 |
| 46 | Procyanidin B2 | 29106‐49‐8 | C30H26O12 | 0.72 | 49.809 |
| Total flavonoid | 12,848.900 | ||||
| Total free flavonoid | 5659.3911 | ||||
| Total conjugated flavonoid | 7189.5085 | ||||
| Chalcone | 1.950 | ||||
| Dihydrochalcone | 1.326 | ||||
| Dihydroflavonol | 760.522 | ||||
| Dihydroflavone | 89.985 | ||||
| Flavonol | 3006.509 | ||||
| Flavone | 592.597 | ||||
| Flavane | 3004.675 | ||||
| Isoflavone | 5391.335 | ||||