FIGURE 4.

Acrylamide influenced the protein level of P2X7. (a) The protein level of P2X7 was determined by western blot after cells were treated with different concentrations of acrylamide for 24 h. (b) Cell viability was measured by the MTT (3‐[4,5‐Dimethylthiazol‐2‐yl]‐2,5‐diphenyltetrazolium bromide) assay after being treated with A 438079 for 30 min. (c) The protein level of P2X7 was determined by western blot after cells were cotreated with A438079 (10.0 μmol/L) and acrylamide (3.0 mmol/L). The results were presented as mean ± SD (standard deviation) (n = 3). *p < .05 and **p < .01 vs. Control, #p < .05 and ##p < .01 vs. Acrylamide (3.0 mmol/L).