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. 2022 Sep 27;11(1):443–457. doi: 10.1002/fsn3.3076

FIGURE 6.

FIGURE 6

Acrylamide induced oxidative stress via P2X7 regulation in RAW 264.7 cells. (a) a) Cells were exposed to acrylamide (4.0 mmol/L) for 30 min followed by incubation with DCFH‐DA (2',7'‐Dichlorodihydrofluorescein diacetate) at 37°C for 30 min, and then were observed by a fluorescence microscope (scale bar =50 μm). b) Quantitation of reactive oxygen species (ROS) was determined by using a Varioskan flash microplate reader after cells were treated with various concentrations of acrylamide for 24 h. (b) The level of ROS was measured by a Varioskan flash microplate reader after cells were treated with A438079 (10.0 μmol/L) and acrylamide (4.0 mmol/L). (c) The protein levels of inducible nitric oxide synthase (iNOS) and heme oxygenase‐1 (HO‐1) were determined by western blot after cells were incubated with different concentrations of acrylamide for 24 h. (d) The expression of iNOS and HO‐1 was determined by western blot after cells were incubated with A 438079(10.0 μmol/L) and acrylamide (3.0 mmol/L), the internal reference (β‐actin) was the same as Figure 4c. The results were presented as mean ± SD (standard deviation) (n = 3). *p < .05 and **p < .01 vs. Control, #p < .05 and ##p < .01 vs. Acrylamide (3.0 mmol/L).