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. 2023 Jan 12;14:173. doi: 10.1038/s41467-023-35798-5

Fig. 3. Prolonged clenbuterol treatment does not affect skeletal muscle GLUT4 translocation or mTORC2 activation.

Fig. 3

A GLUT4 content at the myocellular cell membrane (arb. units). B Total GLUT4 content (arb. units). C GLUT4 content in the myocellular cytosol (arb. units) D Representative images of GLUT4 immunohistochemistry staining for three participants, scale bar 50 µm. E Phosphorylation of mTORS2481 as a marker of mTORC2 activation (arb. units). Both samples of a participant were run on the same blot for comparison. All data were analyzed by means of a two-sided Wilcoxon signed-rank test. A–D n = 9 per group, E n = 10 per group. P = placebo, Cl = clenbuterol. Data are presented as mean ± SEM. Source data are provided as a Source Data file.