a. Frequency of CD68−CD169+ macrophages in LLN (n=21) and MLN (n=19) over age. Linear regression with Pearson correlation (two-tailed). Pearson r, *P < 0.033, **P < 0.005 and ***P < 0.001. Data presented as the mean ± 95% CI. b. Expression of functional markers CD80/86, CD36, and CD209 expression on CD68−CD169+ macrophages over age by flow cytometric analysis of LLNs (n=21, left column) and MLNs (n=19, right column). Linear regression with Pearson correlation (two-tailed). Pearson r, *P < 0.033. Data presented as the mean ± 95% CI.