Skip to main content
. Author manuscript; available in PMC: 2023 Jun 1.
Published in final edited form as: Nat Med. 2022 Nov 21;28(12):2622–2632. doi: 10.1038/s41591-022-02073-x

Extended Data Fig. 6. Particulate-containing CD68+CD169− macrophages induce an immunoregulatory environment in the LLNs.

Extended Data Fig. 6.

a. Confocal image of human LLNs stained for CD68, CD169, and Arginase-1 from donors of indicated ages. Arrows show representative CD68+CD169− macrophages with particulates (magenta) or without particulates (orange). Representative images were taken from 2-6 donors (2-4 images/donor) per age group (≤39,40-64 and ≥65 yrs). Scale bar: 20 um. b. Graph shows paired frequencies of Arg-1+CD68+CD169− macrophages with or without particulates calculated using Imaris software. Significance assessed with repeated measures ANOVA. Significance for ≤39yrs P= 0.059, 40-64 years P=0.0088, ≥65 years P=0.9969. Data are from 13 donors (2-4 single images for each donor). c. Graph shows frequency of Arg-1+CD68+CD169− macrophages with or without particulates over age calculated using Imaris software. (n=13). Linear regression with Pearson correlation (two-tailed). Pearson r, n.s. Data presented as the mean ± 95% CI. d. Confocal image of human LLNs stained for CD68, CD169, and IL-6 from donors of indicated ages. Arrows show representative CD68+CD169− macrophages with particulates (magenta) or without particulates (orange). Representative images were taken from 4-5 donors (2-4 images/donor) per age group (≤39,40-64 and ≥65 yrs). Scale bar: 20 um. e. Graph shows paired frequencies of IL-6+CD68+CD169− macrophages with or without particulates calculated using Imaris software. Significance assessed with repeated measures ANOVA. Significance for ≤39yrs P= <0.0001, 40-64 years P=0.0037, ≥65 years P=0.0002. Data are from 14 donors (2-4 single images for each donor) f. Graph shows frequency IL-6+CD68+CD169− macrophages with or without particulates over age calculated using Imaris software. (n=14). Linear regression with Pearson correlation (two-tailed). Pearson r, n.s. Data presented as the mean ± 95% CI.