Fig. 4.

CTPS2 promoted CLL through participating in Gln and CTP metabolism. A The addition of exogenous CTP rescued the shCTPS2 induced inhibition of CLL proliferation. B, C The overexpression efficacy of CTPS2 mRNA and protein was validated in EHEB and primary CLL cells after plasmids transfected. D, E CTPS2 upregulation enhanced the proliferation of EHEB and CLL primary cells. F CTPS2 enforcement impeded the apoptosis of CLL cells. G, H CTPS2 overexpression could partially rescued the negative effect of Gln-defection on CLL cells’ survival. I Comparable rescued effects on apoptosis were achieved by exogenous supplement of CTP under Gln defection. Each experiment was repeated three or more times independently. *p < 0.05, **p < 0.01, ***p < 0.001