Fig. 1.

Presence of ILCs in the ischemic stroke lesion. A Coordinates of the lesion on the brain of photothromobotic (PT) mouse model at 2.5 mm posterior to bregma and 2.0 mm lateral to midline with 1 mm diameter. B Nissl staining on the vibratome section to indicate the lesion in the stroke brain at P2. Data represent n = 4 mice. C Immunofluorescence on the vibratome sections of stroke brain at P10. Since the markers of ILCs, NKp46, KLRG1 and RORγt are also expressed by CD3⁺ non-ILCs, we used CD3 to distinguish NK/ILC1s from NKT cells, ILC2s from regulatory T cells (Tregs) and NKp46⁺ ILC3s from Th17 cells. CD3⁻NKp46⁺ cells (NK, ILC1 or NKP46⁺ ILC3, white arrows). Data represent n = 4 mice. D CD3⁻KLRG1⁺ cells (ILC2, white arrows). Data represent n = 4 mice (E) CD3⁺RORγt⁺ cells (Th17, green arrows) and CD3⁻RORγt⁺ cells (ILC3, white arrows). Data represent n = 4 mice