FIG. 1.

Schematic of the predicted protein coded by PfRH3. In the diagram, 1 cm represents 200 amino acids. The cross bars represent shifts in the reading frame. SS, signal sequence; TM, transmembrane domain. The homology region (HR) represents an approximately 200-amino-acid fragment with the most similarity to the PvRBP1 and -2 and Py235 proteins. Two regions spanning the frameshifts that were amplified by RT-PCR are shown. Fragments A, B, and C represent regions of the gene used as probes. Dashed lines represent constructs used to make the GST fusion proteins RH3-1, RH3-2, and RH3-4. Dotted lines represent constructs X and Y used for in vitro translation.