FIG. 6.

Mouse anti-PfRH3 antibodies recognize proteins derived from PfRH3 and immunoprecipitate a 200-kDa protein from metabolically labeled parasites, but this is not the protein coded by the gene. (A) Lanes X and Y, in vitro-translated products derived from PfRH3 constructs X and Y, that overlap fragment RH3-1 (see Fig. 1); lanes Xi and Yi; in vitro-translated products from constructs X and Y that are immunoprecipitated by mouse anti-RH3-1 antibodies. In vitro-translated products from unrelated proteins or different fragments of PfRH3 were not immunoprecipitated by anti-RH3-1 antibodies (data not shown). (B) Immunoprecipitated proteins from metabolically labeled 3D7 schizonts using a mouse polyclonal anti-GST serum (αGST) or anti-RH3-1 antibodies (αPfRH3-1). (C) Two-dimensional maps of tryptic peptides derived from (i) in vitro-translated product X (band i) or (ii) the 200-kDa parasite protein immunoprecipitated with anti-RH3-1 antibodies from a schizont pellet.