Table 1. Primers and Locations of 17 Overlapping Truncated ALV-Kgp85 Gene Fragments.
| primer
sequences (5′–3′) |
|||
|---|---|---|---|
| number | forward | reverse | location (aa) |
| gp85-1-1 | GTCCCTATGTTGGATGAGC | AGCTACGCATCCCCCG | 158–193 |
| gp85-1-2 | CAGATCCCTAACGTGGC | GCAATTACACAGAGCCGTTTACG | 183–225 |
| gp85-1-3 | CTCCTCCTCCGCCGC | AGCGAGGACCTGTCTGT | 214–260 |
| gp85-2-1 | CTCCTCCTCCGCCGC | TAGCTTCCAAAATCTGTAACCATA | 214–251 |
| gp85-2-2 | CTCCTCCTCCGCCGC | ACCGCAGTACTCACTCCC | 214–242 |
| gp85-2-3 | CTCCTCCTCCGCCGC | TGTCTATCTGCTGTCACC | 214–232 |
| gp85-2-4 | GAGCCGTTTACGCTGGTGA | AGCGAGGACCTGTCTGT | 222–260 |
| gp85-2-5 | GATAGACACAATCTTTTTAGGG | AGCGAGGACCTGTCTGT | 230–260 |
| gp85-2-6 | TGCGGTACATATGGTTACAGA | AGCGAGGACCTGTCTGT | 241–260 |
| gp85-3-1 | CTCCTCCTCCGCCGC | GCGGCGGAGGAGGAG | 214–218 |
| gp85-3-2 | CTCCTCCTCCGCCGCAAT | ATTGCGGCGGAGGAGGAG | 214–219 |
| gp85-3-3 | CTCCTCCTCCGCCGCAATTAC | GTAATTGCGGCGGAGGAGGAG | 214–220 |
| gp85-3-4 | CTCCTCCTCCGCCGCAATTACACA | TGTGTAATTGCGGCGGAGGAGGAG | 214–221 |
| gp85-3-5 | CTCCTCCGCCGCAATTACACA | TGTGTAATTGCGGCGGAGGAG | 215–221 |
| gp85-3-6 | CTCCGCCGCAATTACACA | TGTGTAATTGCGGCGGAG | 216–221 |
| gp85-3-7 | CGCCGCAATTACACA | TGTGTAATTGCGGCG | 217–221 |
| gp85-3-8 | CGCAATTACACA | TGTGTAATTGCG | 218–221 |