Primers used in this study | ||
---|---|---|
Primer name | Primer sequence | Primer purpose |
bet‐1 qPCR F | CCAACCCGGGAAT GGTGAAGAC | Forward primer to RT‐PCR/qPCR bet‐1 without overlapping with RNAi sequence |
bet‐1 qPCR R | CCATCATTTCCATT TGGTTTGACAACG | Reverse primer to RT‐PCR/qPCR bet‐1 without overlapping with RNAi sequence |
mys‐1 qPCR F | CAGATCATGTTCT AGCAACAACG | Forward primer to RT‐PCR/qPCR mys‐1 without overlapping with RNAi sequence |
mys‐1 qPCR R | GATAGCGTAAGCT TTTCGGTG | Reverse primer to RT‐PCR/qPCR mys‐1 without overlapping with RNAi sequence |
tbas‐1 qPCR F | TCAACACTGCCAT CGCCGCC | Forward primer to RT‐PCR/qPCR tba‐1 loading control |
tba‐1 qPCR R | TCCAAGCGAGACC AGGCTTCAG | Reverse primer to RT‐PCR/qPCR tba‐1 loading control |