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. 2022 Dec 22;22(1):e13752. doi: 10.1111/acel.13752

FIGURE 3.

FIGURE 3

GDF15 knockdown changes SASP composition of HSDF and reduces epidermal thickness in 3D skin equivalents. HSDF were transduced with lentiviral vectors carrying GDF15 shRNA or control shRNA and grown under selection. (a) The percentage of SA‐β‐Gal‐positive cells was estimated. (b) Skin equivalents containing either GDF15KD or control HSDF were stained with H&E. (c) Epidermal thickness [μm] was measured using ImageJ software. Data present mean values ± SD, n = 4; 50 to 55 epidermal sites (excluding stratum corneum) were measured per skin equivalent. N = 9 (3 different donors); (c–e) relative mRNA levels were estimated by qRT‐PCR in control and GDF15KD HFF for MMP1 (d) and IL‐6 (e). Data present mean values ± SD, n = 3; statistical analysis was calculated using t‐test (*p < 0.05, **p < 0.01, ***p < 0.001)