Figure 4.
Verification of Tps1A active site motifs. (a) Alignment of amino acid sequences of the two conserved aspartate-rich motifs of the UbiA prenyltransferase family and the motifs of UbiA-like TPSs. (b) SPME-GC-MS analysis of yeast expressing the tps1A mutants D100A, D215A, D218A, D222A and Y155A. TIC, total ion content. (Online version in colour.)