Table 2.
A subset of metabolic pathways and select metabolites enriched in TCDD exposed fish in comparison to their DMSO counterparts. P-values for each m/z feature are listed in Table S2. Mode = ionization mode in which compounds were detected, m/z and Compound Match = a subset of the enriched metabolites in that pathway, all annotations are Level 3, based on the Schymanski Scale (Schymanski et al., 2014). Regulation = the directionality of the fold change for each feature over the three time points.
| Pathway | Mode | m/z | Compound Match | Regulation |
|---|---|---|---|---|
| Glycosylphosphatidylinositol (GPI)-anchor biosynthesis | + | 577.5195 | Cytidine diphosphate ethanolamine | Up |
| Xenobiotics metabolism | + | 179.0702 | 1,2-Dihydroxy-3,4-epoxy-1,2,3,4-tetrahydronaphthalene | Up |
| + | 211.2054 | Myristic acid | Down | |
| − | 138.0197 | 4-Nitrophenol | Up | |
| − | 161.0639 | 1,2-Dihydronaphthalene-1,2-diol | Up | |
| − | 164.9984 | Thiodiacetic acid sulfoxide | Down | |
| Tryptophan metabolism | + | 160.0604 | 2-Aminomuconate semialdehyde | Down |
| + | 106.029 | 3-Pyridinecarboxylic acid | Down | |
| − | 160.0404 | Quinoline-4,8-diol | Up | |
| − | 179.0211 | alpha-d-Mannose | Up | |
| − | 88.0406 | l-Alanine | Down | |
| − | 156.0303 | 2-Aminomuconate | Down | |
| Bile acid biosynthesis | + | 577.5195 | Cytidine diphosphate ethanolamine | Up |
| − | 429.2987 | 7alpha-Hydroxy-3-oxo-4-cholestenoate | Up | |
| − | 73.0296 | Propionic acid | Up | |
| − | 403.3579 | 3a, 7a-Dihydroxy-5b-cholestane | Up | |
| − | 433.332 | 3a,7a-Dihydroxycoprostanic acid | Down | |
| − | 365.2341 | Tetrahydrocortisol | Down | |
| Androgen and estrogen biosynthesis and metabolism | − | 289.1938 | 5-Androstenediol | Up |
| C21-steroid hormone biosynthesis and metabolism | − | 289.2172 | Androsterone | Down |
| − | 289.2382 | Dihydrotestosterone | Down | |
| − | 315.2328 | Pregnenolone | Up | |
| − | 315.2538 | 20a-Dihydroprogesterone | Up | |
| − | 329.2332 | Deoxycorticosterone | Down | |
| Glutamate metabolism | − | 88.0406 | l-Alanine | Down |
| − | 139.9991 | Carbamoyl phosphate | Down | |
| − | 145.0143 | Oxoglutaric acid | Down | |
| − | 146.0459 | l-Glutamic acid | Down | |
| − | 87.0089 | Pyruvic acid | Down | |
| Tyrosine metabolism | + | 102.0339 | Indole-5,6-quinone | Down |
| − | 101.0021 | Acetoacetic acid | Up | |
| − | 175.0345 | Ascorbate | Up | |
| − | 199.0361 | 4-Maleylacetoacetate | Up | |
| − | 115.0038 | Fumaric acid | Down | |
| − | 97.976 | Sulfate | Down | |
| Chondroitin sulfate degradation | − | 149.0457 | d-Xylose | Down |
| − | 261.1858 | alpha-Santalyl acetate | Down | |
| − | 97.976 | Sulfate | Down | |
| − | 220.1467 | N-Acetyl-d-galactosamine | Down | |
| Vitamin A (retinol) Metabolism | − | 283.1772 | 9-cis-Retinal | Down |
| − | 283.1913 | Retinal | Down | |
| − | 346.0557 | Adenosine monophosphate | Down | |
| − | 283.2091 | 11-cis-Retinaldehyde | Down |