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. 2023 Jan 9;11:e14608. doi: 10.7717/peerj.14608

Figure 4. Effect of miRNA-let-7b-5p on the migration and invasion of lung cancer cells.

Figure 4

(A) RT-PCR was used to detect expression levels of miRNA-23a-3p, miRNA-486-5p, miRNA-30a-3p, and miRNA-let-7b-5p in the HLF-A, A549 and H1299 cell groups, all of which were significantly lower in lung cancer cells. (B) Compared to M0-Exos, the corresponding four miRNAs abnormalities were found in M1-Exos with high expression. MTT (C), scratch (D), and Transwell (E) assays were used to detect the cell viability, migration, and invasion abilities of the four groups. (F) Flow cytometry was used to detect the apoptosis of each group. (G) Western blot was used to detect the proliferation-related protein of PCNA, KI67, Bax, and Bcl-2 expression. * P < 0.05, ** P < 0.01, *** P < 0.001.