FIG. 1.

(A) Comparison of biotinylated and nonbiotinylated surface proteins of adult S. mansoni solubilized with Triton X-100 and separated using streptavidin (strept)-agarose. Proteins were then probed with peroxidase-labeled streptavidin (A). The left lane (unbound) contains proteins that were not precipitated with streptavidin-agarose, while the right lane (bound) contains biotinylated surface proteins that were precipitated. (B) Western blot of the extracts in panel A probed with antiserum raised to rSj-Pmy (α-pmy), showing that Pmy is exposed to labeling procedures on the surface of live parasites. (C) Western blot of biotinylated and nonbiotinylated surface proteins of S. mansoni schistosomula precipitated with streptavidin-agarose and probed with α-pmy serum. Here and in succeeding figures, positions of size markers are shown in kilodaltons at the left.