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Biotinylated and nonbiotinylated surface proteins of adult S. mansoni were solubilized with Triton X-100 shortly after removal from the host (0 h) or after 24 h culture of in vitro after removal from the host (24 h). Surface-labeled proteins were precipitated using streptavidin-agarose and probed with antiserum to rSj-Pmy (A) or to murine IgG (B). The position of the light chain of IgG is shown at the right.
