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. 2001 Jun;69(6):3646–3651. doi: 10.1128/IAI.69.6.3646-3651.2001

FIG. 3.

FIG. 3

FIG. 3

(A) Detection of a 97-kDa biotinylated surface protein of adult S. mansoni that bound to human Fc-Sepharose, (seph). Extracts were obtained from parasites that were labeled after removal from the host (0 h) or after 24 h of culture in vitro (24 h). The blot was probed with peroxidase-labeled streptavidin. (B) Binding of a 97-kDa biotinylated protein from schistosomula to Fc. (C) Demonstration that the bound protein from panel A (24 h) is recognized by antiserum to rSj-Pmy (α-pmy) but not by antiserum to another tegument protein, Sj22.6 (α-Sj 22.6). Depletion of Pmy from biotinylated surface extracts by immunoprecipitation using anti-Pmy serum and protein G-Sepharose prior to incubation of the extract with Fc-Sepharose resulted in an absence of any Fc-BP detected using streptavidin-peroxidase (D). Pmy eluted from Fc-Sepharose in the presence of 2 M but not 1 M NaCl, as detected using streptavidin peroxidase (E). (F) Binding of recombinant Pmy from S. mansoni (kindly provided by C. King) but not of bovine serum albumin (BSA) control to Fc-Sepharose.