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Adsorbed murine immunoglobulins and associated complexes were precipitated from surface extracts of adult S. mansoni by using protein G-Sepharose, and bound proteins were probed by Western blotting with antiserum to recombinant Pmy (α-pmy) or recombinant Sj22.6 (α-Sj22.6) as a control surface protein. Reactivity of the murine immunoglobulin heavy chain with the anti-rabbit IgG secondary antibody is highlighted.
