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. 2022 Nov 15;2(12):100217. doi: 10.1016/j.xgen.2022.100217

Figure 1.

Figure 1

Genome-scale sgRNA delivery in a single mouse liver

(A) Lentiviral vectors for U6-driven expression of an sgRNA and hepatocyte-specific expression of a fluorescent reporter (mCherry or mTurq2).

(B) Images of endogenous mCherry and mTurq2 fluorescence in livers from mice 4 days after injection with an equal mixture of sgAAVS1-mCherry and sgAAVS1-mTurq2 lentiviruses. Livers were counterstained with phalloidin (green) to label actin. Scale bars, 100 μm.

(C) Percentage of mCherry-, mTurq2-, and double-positive hepatocytes in livers from mice 4 days after injection with an equal mixture of sgAAVS1-mCherry and sgAAVS1-mTurq2 lentiviruses. Error bars indicate standard deviation. n = 3 mice per dose and 200 hepatocytes per mouse.

See also Figure S1.