Temporally controlled protein depletion in the mouse liver
(A) Scheme for inducing protein depletion in LSL-Cas9 mice.
(B) Images of livers from LSL-Cas9 mice injected with sgMaob-mCherry followed by PBS or AAV-Cre immunostained for mCherry (magenta), MAO-B (green), and actin (blue). Scale bars, 45 μm.
(C) Cytoplasmic MAO-B intensity per μm in mCherry-positive and mCherry-negative hepatocytes from LSL-Cas9 mice injected with sgMaob-mCherry followed by PBS or AAV-Cre. Closed and open circles represent values from male and female mice, respectively. n = 1 male and 1 female mouse per condition and 25 cells per mouse.
See also Figure S2.