FIG. 2.
Construction of pBJG1-A, a suicide plasmid vector carrying a 969-bp fragment of ompP1 inactivated by insertional mutagenesis with a Kmr cassette. All depicted plasmids and constructs are to the scale noted at bottom right. Ap, ampicillin resistance; Tc, tetracycline resistance; Km, kanamycin resistance; ompP1′ and ompP1", ompP1 fragments of the larger 969-bp ompP1 fragment insertionally inactivated with a Kmr cassette at the indicated, unique BglII site. Note that products of two of the plasmid digests shown appear as base pair size markers in Fig. 4A, lanes 1 and 2. This includes (i) the HindIII/PstI digest of pFRG100 yielding five fragments among which is the 969-bp ompP1 fragment and (ii) the BamHI digest of pUC4K yielding the 1,264-bp Kmr cassette as well as the 2,650-bp plasmid backbone.