FIG. 3.

Western blot analysis of outer membrane preparations from the ompP1⁺ strains wt BCH-1 (lanes 1 to 4) and wt BCH-3 (lanes 5 to 8), and the putative mutant ompP1 BCH-3 Km^r transformant (lanes 10 to 13). AD4 MAb and serum from a chinchilla immunized with a P1_BCH-1-enriched preparation were used to probe unheated (20°C) and heated (100°C) outer membrane preparations. P1 and ΔP1, non-heat-modified and heat-modified forms of OMP P1, respectively (25). Molecular mass standards appear in lane 9.