TABLE 1.
Alignment of four variable regions of OMP P1 from 42 phylogenically diverse typeable H. influenzae and NTHI isolatesa
| P1 typeb | Strain | Sequence of variable regionc:
|
Sd | Loce | |||
|---|---|---|---|---|---|---|---|
| I | II | III | IV | ||||
| 85 107 | 198 232 | 281296 | 427440 | ||||
| 1 | BCH-1 | DSYAIISDS---IKVTNDGSASA | LIADTVKDN-QVKNTLTVQQEPLKFIDKYLPSKDT | LEANVIKASKTGDLTL | KTIGDKRSLALSTT | NT | USA |
| BCH-3 | DSYAIISDS---MKVTNDGSASA | LIADTVKDN-QVKNTLTVQQEPLKFIDKYLPSKDT | LEANVIKASKTGDLTL | KTIGDKRSLALSTT | NT | USA | |
| 3-H-1552 | DSYAIISDS---IKVTNDGSASA | LIADTVKDN-QVKNTLTVQQEPLKYINKYLPSKDT | LEANVINASKTGDLTL | KTIGDKRSLALSTT | NT | USA | |
| 17-H-783 | DSYAIISNS---IKVTNDGSASA | LIADTVKDS-QVKTTLIVQQEPLKSIDKYLPSKDT | LEAEAIKAGKKGXLTL | KTIGDARSLALNTT | NT | USA | |
| 21-H-1328 | NSYAIISNS---IKVTNDGSASA | LIADTVKDS-QVKTTLIVQQEPLKSIDKYLPSKDT | LEAEAIKAGKKGDLTL | KTIGDARSLALNTT | NT | USA | |
| 27-H-1433 | DSYAIISNS---IKVTNDGSASA | LIADTVKDS-QVKNALIVQQEPLKSIDKYLPSKDT | LEAXAIKAGKKGDLTL | KTIGDARSLALNTT | NT | USA | |
| 2 | 200 | NSDAIITSTSSGVKTIKHGSASQ | IIVDSLKDN-QVQTALSVQKEPLKYLHKYLPSKDT | VEADVIKAGKKGDLTL | KTIGDERSLALNTT | NT | Finland |
| 218 | NSDAIITSTSSGVKTIKHGSASQ | IIVDSLKDN-QVQTALSVQKEPLKYLHKYLPSKDT | VEADVIKAGKKGDLTL | KTIGDERSLALNTT | NT | Finland | |
| 667 | NSDAIITSTNSGVKTIKHGSASQ | IIVDSLKDN-QVQTALSVQKEPLKYLHKYLPSKDT | VEADVIKAGKKGDLTL | KTIGDERSLALNTT | NT | Finland | |
| 199 | NSDAIITSTSNGVKTIKHGSASQ | IIVDSLKDN-QVQTALSVQKEPLKYLHKYLPSKDT | VEADVIKAGKKGDLTL | KTIGDERSLALNTT | NT | Finland | |
| 3 | 7416 | DSSAIISNM---IKATKDGSASE | IIADSVKDN-QVKTALTVQQEPLKFLDKYLPSKDT | VEANVIKAGKKGDLTL | KTIGDERSLTLNTT | a | Kenya |
| Rd | TSHATIITSSSGIKAIEGGSASA | IIADSVKDN-QVKTALTVQQEPLKFLDKYLPSKDT | VEANVIKAGKKGDLTL | KTIGDERSLTLNTT | d | USA | |
| 4 | 7004 | T--ASIATTK--MNSAKYGSASE | IIANSVNDT-QVKTALSVLAPPLKGLDQNLPSKDK | VEANVIKEGKKGNLTL | KTIGDKRTLTLNTT | b | Holland |
| 6094 | T--ASIATTK--MNSAKYGSASE | IIANSVNDT-QVKTALSVLAPPLKGLDQNLPSKDK | VEANVIKEGKKGNLTL | KTIGDKRTLTLNTT | b | UK | |
| 6107 | T--ASIATTK--MNSAKYGSASE | IIANSVNDT-QVKTALSVLAPPLKGLDQNLPSKDK | VEANVIKEGKKGNLTL | KTIGDKRTLTLNTT | b | UK | |
| 7109 | T--ASIATTK--MNSAKYGSASE | IIANSVNDT-QVKTALSVLAPPLKGLDQNLPSKDK | VEANVIKEGKKGNLTL | KTIGDKRTLTLNTT | NT | Sweden | |
| ATCC9795 | T--ASIATTK--MNSAKYGSASE | IIANSVNDT-QVKTALSVLAPPLRGLDQNLPSKDK | VEANVIKEGKKGNLTL | KTIGDKRTLTLNTT | b | ||
| 161 | T--ASIATTR--MNSAKYGSASE | IIANSVNDT-QVQTALSVLAPPLKGLDQNLPSKDK | VEANVIKEGKKGNLTL | KTIGDKRALTLNTT | NT | Finland | |
| OT-9 | T--ASIATTK--MNSAKYGSASE | IIANSVKDT-QVKTALSVLAPPRTALPQNLPSKDK | VEANVIKEGKKGNLTL | KTIGDKRALTLNTT | NT | Canada | |
| 5 | 1363 | NSYATITSASSGVRTIKHGSASQ | IIVETI-DN-QVNTALSALQEPFRDLKKHLPSKDK | LEANVIKAGKKGDLTF | KTIGEPRALTFNTT | NT | Finland |
| OT-81 | NSYATITSASSGVRTIKHGSASQ | IIVETI-DN-QVNTALSALQEPFRDLKKHLPSKDK | LEANVIKAGKKGDLTF | KTIGEPRALTFNTT | NT | Canada | |
| 6 | 9-H-1194 | TSHATIVTSSSGVRAIKDGSASA | ILAESVNDDDQVKGALLTLSEPFKNLNTHLPSKDK | LEANVIKAGKKADLTF | KTIGEQRSLTFDTT | NT | Canada |
| 7 | MinnA | TSYAQIITNQIGMKAIKDGSASQ | LIADSVKDN-QITSALSTQQEPFRDLKKYLPSKDK | LEANVIKEGKKGNLTF | KTIGDKRTLTLNTT | b | USA |
| 8 | 1071 | ASSVIISNM---VKATQDGSASA | TISDSVNDS-QLTGVLSTLAKPFKELNKHLPSKDK | LEAGVIGAGKTGNLTL | KTIADKSGLPLTST | NT | Finland |
| 9 | BCH-2 | ASSAIVNSA---MKATKEGSASQ | IIVDTVNDD-QVKRGLTVLQPPYKTLDKYLTSKDK | LEAGVIGAGKTGNLTF | KTIGEKSGLPVTAT | NT | USA |
| 10 | LO-127 | PSSVTTNST---MKTTMDGSASE | VIVDIVNDT-QIKXALAALSEPYKDFPNYLTSKDK | VEANVIKAGKKGDLTL | NTIGKESGLPLTTT | NT | Canada |
| 11 | 5 | TSSA--TPANSVMTAIRYGSASA | IIEGTVNDD-QIKRALTTQPKPYKDFPQHLSSKDK | LEAGVIGAGKKGDLTL | KAIGEKRSLTFDTT | NT | USA |
| 12 | 1512A | SVSASITGAS--VKITRNGSASQ | LIADTVNDN-QIKQALSTQQTTLRDLPKHLTSKDK | LEAEAIKAGKKGDLTL | KAISQA-TLTLDTT | NT | USA |
| 49 | DVRASITGVS--VKTTKNGSASQ | IIANTVNDN-QVKLPLSTQPETLRDLHKHLPSKDK | LEAEAIKAGKKGDLTL | KAITKS-VLTLDTT | NT | USA | |
| 13 | 1-H-1085 | KCYASITGIS--VKTTKNGSASA | LIVDSV-EIARNGQAFTVLGEPLKTIGQYLTSKDK | LGAGVIKAGKKGDLTL | ETISQA-QLTLNTT | NT | USA |
| 14 | 6181 | E--AFIATLK--MKTTKNGSASE | IIADTV-KISQG--AFTVGSQEDKAIPKYLTSKDK | LEAGAIGAGKKGDLTL | ATIG--KSVILNTT | e | UK |
| 15 | 22-H-1154 | DSYAIISNS---IKVTNDGSASA | IIKESV-ELAKT--AFTVAQDDEKAIPTYLTSKNT | LEAEAIKAGKKGDLTL | KTIGDARSLALNTT | NT | USA |
| 16 | 4-H-1094 | K--SSTKNLQ--MLAAKYGSASQ | IIKESV-ELAKT--AFTVAQDDEKAIPTYLTSKNT | LEAGVIGAGKKGDLTL | KTTG--KTVILNAT | NT | USA |
| 17 | 6255 | D--SSITATT--MRTTKYGSASA | IIAESVKI-AQN--AIKTVNPKDK-ATDYLTSKDK | LEAEVIEAGKKGNLTL | QKAVGGF---ITTT | NT | UK |
| ATCC9833 | D--SSITATT--MRTTKYGSASA | IIAESVKI-AQN--AIKTVNPKDK-ATDYLTSKDK | LEAEVIEAGKKGNLTL | QKAVGGF---ITTT | f | ||
| C-105 | N--SSITATT--MRTTKYGSASA | IIAESVKI-AQN--AIKTVNPKDK-ATDYLTSKDK | LEAEVIEAGKKGNLTL | QKAVGGF---IXTT | NT | Canada | |
| 18 | 2-H-1038 | A--ASITGSS--IKVTNDDVASE | IIADSVKD-PQAKRALETVVPGTK-IPDYLTSKDK | LGKKGIEAGKTGNLTF | QKVADDF---ITAN | NT | USA |
| 19 | 658 | L--SSITSAS--MKTTKYGSASE | IIVRSVKD-SQAQTALKTVVPGTL-IPDLLTSKDK | LGQKSIEAGKTDNLTL | QKVADGL---ITTN | NT | Finland |
| 20 | 13-H-1157 | D--AYLQSGS--MKFTKYGSASQ | IIADSVQD-SQVKPALQAVDPQNK-ATGYLTSKDK | LEAGAIKAGKKGDLTL | QKAAGGH---IITT | NT | USA |
| 21 | 9 | N--ASIKAT---MNMTKYGSASQ | LIADTVQD-QQIQKALKAVDSKTV-IPDYVTSKDK | LEAGVIGAGKKGDLTL | QQV-GGH---IITN | NT | USA |
| 22 | 7424 | A--ASIKATA--MGRTKYGSASA | IIADSIQD-NQIQQALKAVDPQTK-LHNYLTSKDK | LGAKDIVAGKTGDLTF | QQA-GGH---IITT | NT | Kenya |
| 23 | 4-H-1093 | D--ASIKATA--MARTKYGSASQ | IIADSIQD-NQIQQALKAVDPQTK-IHEYLTSKDK | LGKKDIVAGKTGDLTL | KTI-GAH---IITN | NT | USA |
a
Alignment is based on data from references 47 and 61 and this study. Putative surface-exposed loops of OMP P1 correlating with variable regions I to IV are loops 2, 4, 5, and 8, respectively (13, 14, 47).
b
Isolates with the same P1 type have OMP P1 amino acid sequences of ≥95% similarity.
c
Numbers indicate amino acid positions at the beginning and end of a variable region. Dashes, gaps inserted to optimize alignment.
d
S, H. influenzae serotype. NT, nontypeable.
e
Loc, country of strain isolation. USA, United States; UK, United Kingdom.