Table 3.
Kinetic parameters of Lsa45 with nitrocefin and 4-Nitrophenyl acetate (p-NPA).
Substrate | Km (μM) | Vmax (μM/s−1) | kcat (s−1) | kcat/Km (μM−1s−1) |
---|---|---|---|---|
Nitrocefin | 235.0 ±6.40 | 0.5642 ±0.020 | 0.040 ±0.002 | 0.17 |
p-NPA | 849.3 ±15.30 | 3.643 ±0.420 | 0.668 ±0.040 | 0.786 |