Table 3. B. subtilis strains used.
Strain | Relevant genotypea [reference] |
---|---|
AG174 (a.k.a., JH642) | pheA1 trpC2 [58, 66] |
BKE40370 | walJ::erm (168 background) [61] |
CMJ293 | ponA::spc |
CMJ374 | cwlO::spc |
GP891 (a.k.a., LSF625) | recU::cat trpC2 ICEBs1 (168 background) [59] |
JMA222 | pheA1 trpC2 ICEBs10 [57] |
JRL131 | ΔoppA (AG174 background) [67] |
JRL189 | ΔoppB (AG174 background) [67] |
LSF20 | speE::spc |
LSF41 | polA::cat (a.k.a., AB41) |
LSF176 | dnaC30(ts)-mls |
LSF197 | ΔSPß::lox-kan-lox |
LSF198 | ΔPBSX::lox-kan-lox |
LSF200 | recJ::spc |
LSF201 | ΔPBSX::lox-kan-lox (PY79 strain background) [68] |
LSF203 | ΔSPß::lox |
LSF204 | ΔPBSX::lox |
LSF225 | ΔSPß::lox ΔPBSX::lox |
LSF231 | ΔSPß::lox ΔPBSX::lox recJ::spc |
LSF233 | ICEBs10 hrcA::cat |
LSF253 | addBA::kan |
LSF254 | ΔSPß::lox ΔPBSX::lox addBA::kan |
LSF270 | recF::spc (a.k.a., CAL1614) |
LSF274 | dnaC30(ts) recJ::spc |
LSF282 | recJ::cat recF::spc |
LSF284 | recN::[recN-9aa-mNeongreen kan]; referred to as recN-mng kan |
LSF298 | recN::kan |
LSF326 | dnaC30(ts) addBA::kan |
LSF444 | SPß::lox PBSX::lox ycgE::spc |
LSF536 | ΔSPß::lox ΔPBSX::lox recN::kan |
LSF633 | amyE::[PyneA-lacZ cat] |
LSF634 | amyE::[PyneA-lacZ cat] recJ::spc |
LSF635 | amyE::[PyneA-lacZ cat] addBA::kan |
LSF648 | ΔSPß::lox ΔPBSX::lox recJ::spc addBA::kan |
LSF649 | ΔSPß::lox ΔPBSX::lox recN-mng kan recJ::spc |
LSF650 | ΔSPß::lox ΔPBSX::lox recN-mng kan addA::spc |
LSF651 | ΔSPß::lox ΔPBSX::lox recN::kan recJ::spc |
LSF652 | ΔSPß::lox ΔPBSX::lox recN::kan addA::spc |
LSF658 | ΔSPß::lox ΔPBSX::lox recA260 (mls, cat) |
LSF659 | ΔSPẞ::lox ΔPBSX::lox recJ::spc recA260 (mls, cat) |
LSF706 | ΔSPß::lox ΔPBSX::lox recN-mng kan recJ::cat addA::spc |
LSF708 | ΔSPß::lox ΔPBSX::lox recN-mng kan |
LSF709 | addBA::kan recF::spc |
LSF740 | ΔSPß::lox ΔPBSX::lox recN-mng kan recU::cat |
LSF756 | ΔSPß::lox ΔPBSX::lox recN-mng kan recA260 (mls, cat) |
LSF814 | recO::cat |
LSF815 | recO::cat recJ::spc |
LSF816 | ΔSPß::lox ΔPBSX::lox recN-mng kan recF::spc |
LSF817 | recO::cat addBA::kan |
LSF818 | ΔSPß::lox ΔPBSX::lox recN-mng kan recO::cat |
a Unless otherwise indicated, strains are derived from JMA222, and the trpC2, pheA1, and ICEBs10 alleles are not shown.