Fig 4. Phylogenetic analyses of nairoviruses.

Phylogenetic trees were constructed based on the RdRp protein sequences of representative viruses in the Nairoviridae family (A), the S segment of JANV and SGLV (B), and the S segment of BJNV and YCNV (C). All the viruses obtained in ticks here were highlighted in red, and the closest referenced viruses were also highlighted in bold font. In panel B and C, viral strains identified in this study were marked with red-filled circles, while strains found in humans were marked with blue-filled circles. JANV, Ji’an nariovirus; SGLV, Songling virus; TCTV1, Tacheng tick virus 1; SXTV2, Shanxi tick virus 2; HNTV, Henan tick virus; YCNV, Yichun nariovirus; BJNV, Beiji nariovirus; GKTV, Gakugsa tick virus; PTV, Pustyn virus; NWNV1, Norway nairovirus 1; GTV, Grotenhout virus. The accession numbers of the viral sequences are shown in S2 and S3 Tables.