Table 1. Ingredients of the experimental diets.
| Se-adequate (0.15 mg kg-1 Se) |
Se-supplemented (1.4 mg kg-1 Se) |
||
|---|---|---|---|
| Sodium selenate | SeMet | Se-Nuts | |
| Starch (g) | 609.8 | 609.8 | 609.8 |
| Casein (g) | 200 | 200 | 197.5 |
| Cellulose (g) | 50 | 50 | 49.2 |
| Sucrose (g) | 50 | 50 | 48 |
| Soybean oil (mL) | 40 | 40 | 30.5 |
| AIN-93 M Mineral Mix*1 (g) | 35 | 35 | 35 |
| AIN-93 M Vitamin Mix*2 (g) | 10 | 10 | 10 |
| Methionine (g) | 3 | 3 | 3 |
| Choline (g) | 2 | 2 | 2 |
| BHT (g) | 0.2 | 0.2 | 0.2 |
| Selisseo® 2% Se (g) | - | 0.0035 | - |
| Brazil nut (g) | - | - | 14.8 |
| kcal/kg diet | 3759.2 | 3759.2 | 3753.8 |
*1 Mix by Rhoster® Co. Mineral element content (g/kg mix): 357 g of calcium carbonate, anhydrous (40.04% Ca); 250 g of potassium phosphate, monobasic (22.76% P; 28.73% K); 209.806 g of powdered sucrose; 74 g of sodium chloride (39.34% Na; 60.66% Cl); 46.6 g of potassium sulfate (44.87% K; 18.39% S); 28 g of potassium citrate, tri-potassium, monohydrate (36.16% K); 24 g of magnesium oxide (60.32% Mg); 6.06 g of ferric citrate (16.5% Fe); 1.65 g of zinc carbonate (52.14% Zn); 1.45 g of sodium meta-silicate, 9 hydrate (9.88% Si); 0.63 g of manganous carbonate (47.79% Mn); 0.30 g of cupric carbonate (57.47% Cu); 0.275 g of chromium potassium sulfate, 12 hydrate (10.42% Cr); 0.0815 g of boric acid (17.5% B); 0.0635 g of sodium fluoride (45.24% F); 0.0318 g of nickel carbonate (45% Ni); 0.0174 g of lithium chloride (16.38% Li); 0.01025 g of sodium selenate, anhydrous (41.79% Se); 0.01 g of potassium iodate (59.3% I); 0.00795 g of ammonium paramolybdate, 4 hydrate (4.34% Mo); 0.0066 g of ammonium vanadate (43.55% V).
*2 Mix by Rhoster® Co. Vitamin content (g/kg mix): 974.655 g of powdered sucrose; 15 g of vitamin E (all-rac-a-tocopheryl acetate) (500 IU/g); 3 g of nicotinic acid; 2.5 g of vitamin B-12 (cyanocobalamin) (0.1% in mannitol); 1.6 g of Ca pantothenate; 0.8 g of vitamin A (all-trans-retinyl palmitate) (500,000 IU/g); 0.7 g of pyridoxine-HCl; 0.6 g of thiamin-HCl; 0.6 g of riboflavin; 0.25 g of vitamin D3 (cholecalciferol) (400,000 IU/g); 0.2 g of folic acid; 0.075 g of vitamin K (phylloquinone); 0.020 g of D-biotin.