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. Author manuscript; available in PMC: 2023 Aug 1.
Published in final edited form as: FASEB J. 2022 Aug;36(8):e22441. doi: 10.1096/fj.202101627RR

Figure 2. SNAP23 depletion decreases myoblast proliferation.

Figure 2.

C2C12 myoblasts were transfected with a control si-Luciferase (si-Luc) or one of three different si-RNAs targeting Snap23 (#1, #2, and #3). A. Myoblasts and myotubes were treated with BrdU and immunofluorescence assays were performed to calculate the percentage of proliferating (i.e., Brdu-positive) cells. B. The expression of Ki67 was measured by qPCR in myoblasts and myotubes. C-D. The expression of cyclin D1 (CCND1) was measured by western blot experiments in myoblasts (C) and myotubes (D). Results are shown as the mean ± SEM, *p ≤ 0.05 versus mock and si-Luc, Student’s T-test for panels A and B, one-way ANOVA with Bonferroni post hoc test for multiple comparisons for panels C and D, n = 3–5 independent experiments. TUBB2B: beta-tubulin.