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. 2022 Aug 17;33(1):e13113. doi: 10.1111/bpa.13113

FIGURE 3.

FIGURE 3

Fra‐1 regulates apoptosis response in oxygen–glucose deprivation/reoxygenation (OGD/R)‐stressed VSC4.1 neurons. (A,B) CCK‐8‐assisted analysis of cell viability after OGD/R. (C,D) Western blot‐assisted analysis of the relative protein levels of Fra‐1 after transfection with si‐Fra‐1‐1, si‐Fra‐1‐2, si‐Fra‐1‐3 under OGD/R. (E,F) Western blot‐assisted analysis of the relative protein levels of cleaved caspase‐3, Bax and Bcl‐2 in VSC4.1 neurons after transfection with si‐Fra‐1 under OGD/R. (G,I) Western blot‐assisted analysis of the relative protein levels in VSC4.1 neurons after transfection with the plasmid used for Fra‐1 overexpression (OE‐Fra‐1) under OGD/R. (H) Immunofluorescence (IF) assays of cleaved caspase‐3 and Fra‐1 after OGD/R were performed. Scale bar = 100 μm. (J) Quantification of the cleaved caspase‐3 and Fra‐1 fluorescence intensity (INT/mm2). All data represent the mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001.