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. 2022 Aug 17;33(1):e13113. doi: 10.1111/bpa.13113

FIGURE 7.

FIGURE 7

TAK‐242 attenuates S100A8‐induced apoptosis and activation of ERK1/2 and NF‐κB pathways and IL‐1β production, and activated TLR4/NF‐κB and ERK1/2 pathways promote Fra‐1 expression after I/R. (A) Schematic of the experimental design shows the timeline for grouping rats. The rats were intrathecally injected rS100A8, and tissue samples were collected 48 h later. (B) The rats were intraperitoneally injected TAK‐242 30 min before administration of rS100A8 and intrathecally injected rS100A8 before I/R induction. After 48 h of I/R, tissue samples were collected. (C) TUNEL staining of the ventral horn of the spinal cord showing apoptotic cells (n = 3 rats/group). Scale bar = 100 μm. (D) Quantification of TUNEL‐positive cells after I/R. (E,F) Western blot‐assisted analysis and quantification of the protein levels of p‐ERK/ERK, p‐NF‐κB/NF‐κB, IL‐1β, and Fra‐1 (n = 5 rats/group). All data represent the mean ± SEM. **p < 0.01, ***p < 0.001, and ****p < 0.0001.