TABLE 3.

Antibodies included in the study

Antibody	Clone/product code	Concentration	Species/type	Epitope retrieval	Detection/instrument
Hif‐1α	54 BD biosciences	1:100 32 min at 36°C + amplification	Mice monoclonal	HIER: CC1 for 48 min at 100°C	Optiview Bench Mark Discovery
CD44	DF1485 Novus biologicals	1:200 32 min at 36°C	Mice monoclonal	HIER: CC1 for 32 min at 100°C	Optiview Benck Mark Discovery
VISTA	D1L2G Cell Signaling Technology	1:100 32 min at 36°C	Rabbit monoclonal	HIER: CC1 for 48 min at 100°C	Optiview Benck Mark Discovery
B7‐H3	AF1027 R&D systems	1:800 32 min at 36°C	Goat polycloncal	CC1 for 32 min at 100°C HIER:	Optiview Benck Mark Discovery
Iba1	019–19,741 Wako Pure Chemical	1:2000 16 min at 36°C	Rabbit polyclonal	HIER: CC1 for 32 min at 100°C	Optiview Bench Mark Discovery
CD4	SP35 Ventana Medical Systems	Ready‐to‐use 24 min at 36°C	Rabbit monoclonal	HIER: CC1 for 32 min at 100°C	Optiview Bench Mark Discovery
CD8	C8/144B	1:100 32 min at 36°C	Mice monoclonal	HIER: CC1 for 32 min at 100°C	Optiview Bench Mark Discovery
FOXP3	236A/E7	1:40 16 min at 36°C + amplification	Mice monoclonal	HIER: CC1 for 64 min at 100°C	Optiview Bench Mark Discovery

Note: Cell conditioning solution (CC1 Tris‐EDTA buffer, pH 7.8).

Abbreviation: HIER, heat‐induced epitope retrival.