FIGURE 11.

R1‐Pep reduced neuronal death after MCAO. Brain slices prepared from the somatosensory cortex of sham and MCAO treated mice were incubated for 6 h with R1‐Pep and then stained for the presence of neurons using NeuN antibodies (A) or for degenerating neurons using Fluoro‐Jade C staining (B) (scale bars: Overviews, 1 mm; higher magnifications, 50 μm). (A′, B′) Quantification of neurons. The data represent the average of multiple frames (fields of view) per mouse (A′: N = 7 mice per condition, B′: N = 3 for sham and n = 5 for MCAO). Two‐way ANOVA with Tukey's multiple comparison test (ns, p > 0.05; *, p < 0.05; **, p < 0.01). MCAO, middle cerebral artery occlusion.