Skip to main content
. Author manuscript; available in PMC: 2023 Jan 12.
Published in final edited form as: J Vis Exp. 2022 Feb 25;(180):10.3791/63106. doi: 10.3791/63106

Figure 4: Screenshot of the two-photon laser software during image acquisition.

Figure 4:

(A) Ensure to set the pinhole to the highest value (red arrows). Adjust laser wavelengths, gain, power, and offset. Adjust pixel dwell time, the pixel size, averaging, and the zoom. (B) Set the Z volume to 20 μm and Z-step size to 1 μm.