FIG. 2.

(A) Analysis of surface marker expression in uUSC by FACS. A clone of uUSC (p4) was stained with CD markers expressed by mesenchymal as well as hematopeietic stem cells. All MSC markers stained positive, whereas the hematopoitic markers (CD31, 34, and 45) were negative. (B) Immunofluorescence staining for stem cell markers. Immunofluorescent images of uUSC (p3) stained positive (green) for MSC markers (CD44, CD73, CD105, CD133, STRO-1, and SSEA-4) and negative for hematopoietic stem cell markers (CD31 and CD34) are shown. (C) Expression of pericyte markers. Immunofluorescent images of uUSC (p3) stained (green) with pericyte markers (CD146, NG2, and PDGF-rβ). Nucleus was counterstained with propidium iodide (PI, red). Representative cells exhibiting distinct membrane staining are shown by arrows. The inset shows cells stained using control immunoglobulin fraction. Scale bar=50 μm. MSC, mesenchymal stem cell. Color images available online at www.liebertonline.com/tea