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. 2011 Jul 20;17(15-16):2123–2132. doi: 10.1089/ten.tea.2010.0637

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Copyright 2011, Mary Ann Liebert, Inc.

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FIG. 4. — Gene expression analysis of differentiated uUSC. Reverse transcriptase–polymerase chain reaction analysis of uUSC (p5) differentiated into (A) myogenic lineage and (B) uroepithelial lineage on day 14. Specific transcripts were amplified with lineage-specific primers only in the differentiated samples. uUSC, nontreated control; uUSC-SMC, differentiated to myogenic lineage for 14 days; uUSC-UC, differentiated to uroepithelial lineage for 14 days and H₂O is no template control. RNA from SMC and UC serve as positive controls. (C) Real-time analysis of uUSC for myogenic and urothelial-specific transcripts. A single clone of uUSC (p4) was differentiated into SMC and UC by incubation in specific growth factor containing medium for 12 days. At least a twofold increase in all the transcripts was observed. Up, uroplakin; ZO, Zona Occludens; Ecad, epithelial cadherin; UC, urothelial cells.