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. 2011 Jul 20;17(15-16):2123–2132. doi: 10.1089/ten.tea.2010.0637

FIG. 5.

FIG. 5.

(A) Immunofluorescent staining of uUSC for smooth muscle-specific markers. uUSC (p6) were differentiated to SMC lineage by culturing in myogenic medium containing TGF-β1 (2.5 ng/mL) and PDGF-BB (5.0 ng/mL) for 14 days. Nontreated uUSC controls are shown in the top row. Upon differentiation, specific staining was observed with desmin, myosin, and vimentin antibodies. (B) Immunofluorescent staining of uUSC for urothelial-specific markers. uUSC(p6) differentiated to urothelial lineage by culturing in epithelial medium containing epidermal growth factor (30 ng/mL) for 14 days. Nontreated uUSC controls are shown in the top row. Upon differentiation, specific staining or enrichment was observed with the urothelial-specific antibodies. Image scale bar=50 μm. The inset shows cells stained using control immunoglobulin fraction. Color images available online at www.liebertonline.com/tea