Table 6.
Details of Antibodies Used for Immunofluorescence Staining and FACS Analysis
| Primary antibody | Source/catalog no. | Antibody dilution |
|---|---|---|
| Desmin | Santa Cruz/sc23879 | 1:100 |
| Myosin heavy chain | Sigma/M7786 | 1:50 |
| Smooth muscle actin | Sigma/A5228 | 1:100 |
| Vimentin (S-20) | Santa Cruz/sc7558 | 1:200 |
| Uroplakin-Ia | Santa Cruz/sc15173 | 1:50 |
| Uroplakin-III | US Biological/U2735-50 | 1:100 |
| AE1/AE3 | Dako/M3515 | 1:200 |
| Cytokeratin-13 | Novocastra/NCL-CK-13 | 1:50 |
| CD31 | Santa Cruz/sc1506 | 1:50 |
| CD34 | Miltenyi Biotec/130-081-002 | 1:50 |
| CD44 | R&D Systems/BBA10 | 1:50 |
| CD73 | Becton Dickinson/#550257 | 1:50 |
| CD105 | R&D Systems/MAB10971 | 1:50 |
| CD133 | Abcam/ab19898 | 1:50 |
| SSEA-4 | R&D Systems/MAB1435 | 1:50 |
| STRO-1 | R&D Systems/MAB1038 | 1:50 |
| CD146 | Abcam/ab75769 | 1:100 |
| NG2 | Abcam/ab50009 | 1:100 |
| PDGF-rβ | Abcam/ab32570 | 1:100 |
| CD44-FITC | BD Pharmingen/560977 | 1:5 |
| CD105-PerCP-Cy | BD Pharmingen/560819 | 1:20 |
| CD146-PE | BD Pharmingen/561013 | 1:5 |
| CD73-PE | BD Pharmingen/561014 | 1:5 |
| CD90-FITC | BD Pharmingen/555595 | 1:5 |
| CD45-FITC | BD Pharmingen/560976 | 1:5 |
| CD34-FITC | BD Pharmingen/560942 | 1:5 |
| CD31-FITC | BD Pharmingen/560984 | 1:5 |
| CD117 | Santa Cruz/sc13508 | 1:10 |
| CD133 | Abcam/ab16518 | 1:10 |
| SSEA4-FITC | BD Pharmingen/560126 | 1:5 |
| STRO-1 | Abcam/ab102969 | 1:10 |
FITC, fluorescein isothiocyanate; PE, phycoerythrin; PerCP-Cy, peridinin chlorophyll protein-cyanine.