A Coomassie blue-stained reducing SDS-polyacrylamide gel of whole-cell lysate, Triton X-114 partitioning material (TX-114), and Triton X-114 aqueous-phase material from two clones of the 55 lineage, 55-5 and 55-7, as indicated. MAb reactivity refers to whether the clones do (+) or do not (−) bind 4G12 and 3B3. Differences between clones are indicated by asterisks at the right of the protein bands. (B) Western blot analysis on strips containing Triton X-114-partitioned antigen from clones indicated at the top. The primary antibody used is indicated above each strip (α39, rabbit anti-Vpma39 serum; α34, rabbit anti-Vpma34 serum).