FIG. 3.

Alignment of all determined Vpma amino-terminal sequences either by amino-terminal sequencing (Vpma39 and Vpma34) or translated from the DNA sequence (vpmaX and vpmaY) and from two homologous vsp genes from M. bovis. GenBank accession numbers are L81118 for VspA and AF162139 for VspE (23, 24). Amino acids which differ from the top sequence (vpmaX) are shaded. The sequences boxed were used to design the vpma gene-specific oligonucleotide 3AF. The arrowhead indicates the proposed cysteine residues involved in lipid attachment.