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Southern blot analysis of genomic DNA from clones 55-5 (lanes 1), 55-7 (lanes 2), 60-3 (lanes 3), and 60-4 (lanes 4). The membrane was first hybridized with DIG-labeled A3F oligonucleotide (A) and then stripped and hybridized with DIG-labeled vspS-2 oligonucleotide based on the leader sequence of vspA from M. bovis type strain PG45 (B). Restriction digests of the genomic DNA are indicated above the lane sets. Asterisks show DNA fragments that are detected with only one probe.
