FIG. 6.

(A) Clustal W DNA alignment of the 5′ noncoding and coding regions of vspA, vpmaY, and vpmaX. An asterisk represents identity between all three nucleotide sequences, and a dash represents a gap introduced to optimize the alignment. The translated amino acid sequences are aligned above the DNA sequences. A putative ribosomal binding sequence (SD) is overlined, and an 11-nucleotide imperfect inverted repeat is marked by an arrowed line above the sequence. A box encompasses the vspA sequence used to design the leader oligonucleotide vspS-2.