Table 2.
The pair of the primers designed in qPCR.
| Target | Primers | Product |
|---|---|---|
| miR-181a-5p | CGCAACATTCAACGCTGTCGG | — |
|
| ||
| Bcl-2 https://www.ncbi.nlm.nih.gov/gene/596 |
Forward: AGCTGCACCTGACGCCCTT | 147 bp |
| Reverse: ACATCTCCCGGTTGACGCTCT | ||
|
| ||
| Beclin1 https://www.ncbi.nlm.nih.gov/gene/8678 |
Forward: CATGGAGAACCTCAGCCGAA | 172 bp |
| Reverse: ACAGCGTTTGTAGTTCTGACAC | ||
|
| ||
| LC3 https://www.ncbi.nlm.nih.gov/gene/81631 |
Forward: TTCAGGTTCACAAAACCCGC | 163 bp |
| Reverse: TCTCACACAGCCCGTTTACC | ||
|
| ||
| Atg5 https://www.ncbi.nlm.nih.gov/gene/9474 |
Forward: AAGATGTGCTTCGAGATGTGT | 144 bp |
| Reverse: ACTTTGTCAGTTACCAACGTCA | ||
|
| ||
|
β-Actin https://www.ncbi.nlm.nih.gov/gene/60 |
Forward: ACCCTGAAGTACCCCATCGAG | 224 bp |
| Reverse: AGCACAGCCTGGATAGCAAC | ||