Fig. 1.

The effect of phloretin on cell viability. a Phloretin does not decrease RPE cell viability at the studied concentration of 100 µM. b Lipopolysaccharide (LPS) exposure with or without a 1 h pretreatment with 100 µM phloretin (PHL) does not reduce cell viability. c Brightfield microscopy images of cells exposed to vehicle or 10 µg/ml LPS with or without 1 h pretreatment with 100 µM phloretin reveal no changes to cellular morphology. Data are combined from three to nine independent experiments with four parallel samples per experiment and are represented as mean ± SEM. Representative microscopy images are shown. PHL—100 µM phloretin, LPS—10 µg/ml LPS, ns not statistically significant, ****p < 0.0001, Mann–Whitney U-test