Fig. 3.

The uptake of [14C]-Glucose and [14C(U)]-Methyl α-D-glucopyranoside ([14C]-α-MDG) by ARPE-19 cells. a/b The uptake rate of 0.25 μCi/ml [14C]-Glucose (86.2 µM) (a) or of 0.5 μCi/ml [14C]-α-MDG (2.0 µM) (b) measured over 5 or 10 min, respectively, in the absence or presence of 100 µM phloretin (PHL) or 10 µM phloridzin (PHZ). Phloretin, but not phloridzin significantly reduces the glucose uptake rate. (c/d) The uptake rate of 0.25 μCi/ml [14C]-Glucose (86.2 µM) measured for 5 min after 1 h pre-incubation with PHL and LPS, or 24 h incubation with LPS. Glucose uptake was measured in the absence (c) or presence of PHL (d). Removal of phloretin from culture medium prior to glucose uptake rate measurement leads to an increased uptake of [14C]-Glucose. Data are presented as the mean ± SEM of data from three to five independent experiments. ns not statistically significant, *p < 0.05, Mann–Whitney U-test