Fig. 3.

(A) 5’-Cap analysis demonstrates that Peak 1 (middle) and Peak 2 (bottom) materials contain the expected capping structure at the 5’ end, consistent with the capping structure observed in the input BNT162b2 material (top). Relative quantitation of the capped content demonstrates comparable levels of capped/non-capped species across all three samples. (B) Poly(A) tail analysis reveals that the fragment species in Peak 1 (middle) lack the poly(A) tracts that are observed in the BNT162b2 input (top) and intact species enriched in the Peak 2 sample (bottom).