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. 2022 Oct 4;261(2):409–425. doi: 10.1007/s00417-022-05837-w

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Effects of intravitreal application of vehicle ascorbic acid (green, n = 11 eyes), atropine + spiperone (orange, n = 11 eyes), atropine (red, n = 7 eyes), and no injection (yellow, n = 6 eyes) on dopamine and DOPAC content, 2 h after injections. A There were no significant effects on dopamine content in vitreous and retina in spiperone + atropine–injected eyes, compared to atropine alone, but dopamine was clearly increased in the choroid in atropine + spiperone–injected eyes. B Atropine + spiperone injections reduced DOPAC content in the vitreous, compared to atropine alone, or to untreated eyes with no injection at all or to eyes injected with ascorbic acid (green bar). In the retina, DOPAC was elevated both after ascorbic acid and atropine + spiperone injections, compared to atropine alone and non-injected eyes. Two-tailed unpaired Student’s t-tests; *p < 0.05, **p < 0.01. Colors of asterisks match the color of the data column for comparison